Basic Sciences Successful Microbubble Sonothrombolysis Without Tissue-Type Plasminogen Activator in a Rabbit Model of Acute Ischemic Stroke

Background and Purpose—Microbubbles (MB) combined with ultrasound (US) have been shown to lyse clots without tissue-type plasminogen activator (tPA) both in vitro and in vivo. We evaluated sonothrombolysis with 3 types of MB using a rabbit embolic stroke model. Methods—New Zealand White rabbits (n 74) received internal carotid angiographic embolization of single 3-day-old cylindrical clots (0.6 4.0 mm). Groups included: (1) control (n 11) embolized without treatment; (2) tPA (n 20); (3) tPA US (n 10); (4) perflutren lipid MB US (n 16); (5) albumin 3 m MB US (n 8); and (6) tagged albumin 3 m MB US (n 9). Treatment began 1 hour postembolization. Ultrasound was pulsed-wave (1 MHz; 0.8 W/cm) for 1 hour; rabbits with tPA received intravenous tPA (0.9 mg/kg) over 1 hour. Lipid MB dose was intravenous (0.16 mg/kg) over 30 minutes. Dosage of 3 m MB was 5 10 MB intravenously alone or tagged with eptifibatide and fibrin antibody over 30 minutes. Rabbits were euthanized at 24 hours. Infarct volume was determined using vital stains on brain sections. Hemorrhage was evaluated on hematoxylin and eosin sections. Results—Infarct volume percent was lower for rabbits treated with lipid MB US (1.0% 0.6%; P 0.013), 3 m MB US (0.7% 0.9%; P 0.018), and tagged 3 m MB US (0.8% 0.8%; P 0.019) compared with controls (3.5% 0.8%). The 3 MB types collectively had lower infarct volumes (P 0.0043) than controls. Infarct volume averaged 2.2% 0.6% and 1.7% 0.8% for rabbits treated with tPA alone and tPA US, respectively (P nonsignificant). Conclusions—Sonothrombolysis without tPA using these MB is effective in decreasing infarct volumes. Study of human application and further MB technique development are justified. (Stroke. 2011;42:2280-2285.)